马铃薯收获机设计试验研究

张家口地区马铃薯种植面积广,畜力犁收获时效率低,漏收和破损较为严重,外购的马铃薯收获机不适合当地农民的种植方式.为此,设计了马铃薯收获机.该机可实现马铃薯挖掘、薯土分离和薯秧分离.同时,对该机部分关键零件进行了设计,对样机在玉米茬地和马铃薯地的工作性能进行了试验研究.试验结果满足当地农民的使用要求,试验数据对设计多行马铃薯收获机具有参考和借鉴作用.     

Mechanism of Fuzilizhong decoction alleviating inflammatory damage of rats with non-alcoholic fatty liver disease

AIM To observe the effect of Fuzilizhong decoction on the inflammatory damage of non-alcoholic fatty liver disease （NAFLD） rats and to explore its mechanism. METHODS SPF male SD rats were randomly divided into 6 groups： control group, model group, high dose （20 mg·kg^-1·d^-1）, middle dose （10 mg·kg^-1·d^-1）, low dose （5 mg·kg^-1·d^-1） Fuzilizhong decoction group and Yishanfu （30 mg·kg^-1·d^-1）group, 8 rats in each group. A NAFLD rat modelwas established by intragastric administration of fat emulsion for 4 weeks. Then the drug was given for 4 weeks in each treatment group. HE staining was performed to observe the histopathological changes of the rat liver.The serum levels of interleukin-2（IL-2）, IL-6 and tumor necrosis factor-α（TNF-α） were measured by ELISA. The expression of toll like receptor 4（TLR4） and NF-κB p65 in liver tissues at mRNA and protein levels was determined by RT-qPCR and Western bolt,respectively. RESULTS Compared with control group, the inflammatory damage of liver tissue was more serious, the serum levels of IL-2, IL-6 and TNF-α, the mRNA expression TLR4 and NF-κB p65 in liver tissues were significantly increased in model group（P<0.05）. However, compared with model group, the liver pathological changes in each treatment group were significantly relieved, the serum levels of IL-2, IL-6 and TNF-α, the mRNA expression of TLR4 and NF-κB p65 in liver tissues were significantly reduced（P<0.05）.In addition, the changes of TLR4 and p-NF-κB p65 protein levels in liver tissue were consistent with the changes of TLR4 and NF-κB p65 mRNA. CONCLUSION Fuzilizhong decoction attenuates the inflammatory damages of NAFLD in rats by inhibiting TLR4/NF-κB p65 signaling pathway.     

MicroRNA-9-5p mediates sympathetic overactivity by targeting KCNN3 in paraventricular nucleus of rats with T2D

AIM To investigate whether microRNA-9-5p （miR-9-5p） mediates sympathetic overactivity by targeting KCNN3 （potassium intermediate/small conductance calcium-activated channel, subfamily N, member 3） gene,which encoded small-conductance calcium-activated potassium channel 3 （SK3） protein, in paraventricular nucleus （PVN） of rats with type 2 diabetes mellitus （T2D）. METHODS A rat model of T2D was established by high-fat diet combined with intraperitoneal injection of 30 mg/kg streptozotocin. The levels of miR-9-5p and KCNN3 mRNA in PVN were detected by real-time PCR. The relationship between KCNN3 and miR-9-5p was predicted by TargetScan. Recombinant adeno-associated virus （rAAV）-miR-9-5p or KCNN3 were bilaterally microinjected into the PVN to observe the changes in plasma glucose levels and sympathetic drive indicators. The number of FosB and SK3 positive cells was measured by immunofluorescence staining. The protein expression of SK3 was determined by Western blot. The relationship between KCNN3 and miR-9-5p were confirmed by cell transfection and dual-luciferase reporter assay. RESULTS Compared with the rats in diabetes control （DC） group, the blood glucose, sympathetic drive indexes and the level of miR-9-5p in PVN were significantly increased, while the SK3 expression in PVN was obviously reduced in the diabetes mellitus （DM） rats. After microinjecion of rAAV-miR-9-5p in PVN, the sympathetic drive indexes, blood glucose, and the number of FosB-positive cells were increased significantly, but the SK3 protein expression was significantly reduced （P<0.05）. However, up-regulation of KCNN3 in PVN had the opposite effect. These responses were obviously enhanced in DM rats compared with DC rats. The results of cell transfection and dual-luciferase reporter assay demonstrated that miR-9-5p bound to the 3’-UTR of KCNN3 and inhibit its expression. CONCLUSION miR-9-5p was up-regulated in PVN of the rats with T2D, and it may mediate sympathoexcitation by targeting KCNN3.     

桃新品种‘秋露蜜’的选育

‘秋露蜜’是从‘国庆’桃实生后代中选出的浓甜晚熟桃新品种。平均单果重248.3 g,果面鲜红,果肉乳白色,甘甜,品质上等,平均硬度达15.30kg/cm²,可溶性固形物含量19.08%。在晋南地区,果实9月上中旬成熟,果实发育期162 d左右。离核。     

一种新的结合SVM和FNN的多聚焦图像融合算法

产业融合共生是新时期红色旅游的必然选择，红色旅游企业共生系统可分为区域内共生子系统和区域外共生子系统，表现为核心共生、主要共生和跨界共生三种形态，核心共生形态是红色旅游企业共生区别其他企业共生的根本所在。红色旅游企业共生由合作和共识耦合而成，但核心共生形态的形成机理更多地表现为天然性，要求红色旅游企业主动寻求合作，争取与其他共生单元达成共识，促进红色旅游企业共生关系的良性发展。     

北京地区无土栽培越冬茬番茄品种筛选

为了筛选出适宜北京地区无土栽培的越冬茬番茄品种,对从国外引进的"丰收560""迪安娜""玛丽娅""粉妮娜"4个番茄品种进行了品种比较试验,以综合比较各品种的生长指标、果实性状和果实营养品质。结果表明:"迪安娜"综合表现较好,更适于北京地区无土栽培越冬茬生产。     

民和县生态建设工程的现状问题及对策

通过对民和县生态建设工程现状的调查,针对存在的问题,分析了原因,并提出了今后的思路和对策。     

论我国旅游投诉解决机制及其完善——以非诉讼纠纷解决方式为视角

随着我国旅游业规模的不断扩大，旅游体系也日趋完善。在推进旅游产业快速发展的进程中，各种类型的旅游纠纷也随之迅速增加。在目前我国多元化纠纷解决机制日趋完善的背景下，旅游投诉解决机制对于确保旅游业和谐稳步推进有着特殊的功能优势。但鉴于旅游投诉解决机制的固有局限性，需要从明确旅游投诉处理结果的效力、增设旅游投诉解决机制为司法救济的前置程序等方面予以完善。     

我国微生物航天诱变育种的应用及研究进展

航天诱变是近些年发展迅速的一种新型的微生物诱变育种技术。根据国内微生物航天诱变的研究状况,综述了微生物航天诱变的机制以及产生代谢产物的研究进展,提出了其存在的问题,并对微生物航天诱变育种进行了展望。